Tag

Part:BBa_K3581014:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-23)


TorA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 139


Design Notes

HindIII cut sites are flanking the coding region, allowing the signal peptide to be cut out so secretion method is changed from extracellular to inclusion bodies.


Source

Original sequence designed by iGEM 19 Calgary.

References

1. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38